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Anti -alpha Tubulin Mouse mAb

價(jià)格: 在線(xiàn)咨詢(xún)
貨號(hào):
DRF101371
產(chǎn)品規(guī)格:
100 μL
Applications:
Species:
購(gòu)買(mǎi)數(shù)量:
產(chǎn)品詳情 操作說(shuō)明 注意事項(xiàng)

Product Information


Description

alpha Tubulin mouse monoclonal

Protein full name

Tubulin alpha-1A chain

Synonyms

TUBA1A, B-ALPHA-1, LIS3, TUBA3, tubulin alpha 1a,TUBA1B, K-ALPHA-1, tubulin alpha 1b

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse alpha Tubulin

Isotype

IgG2b

Purity

Affinity purification

Subcellular location

Cytoskeleton

Predicted MW.

50 kDa

Observed MW.

55 kDa

Uniprot ID

P68366, P68368




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 2000-1: 4000

lung, brain

IHC

Human, Mouse, Rat

1: 1000-1: 2000


IF

Human, Mouse, Rat

1: 1000-1: 2000

kidney, fibrogenic tumor, brain, lung, kidney



Background


Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. 




Images


Western blot analysis of alpha Tubulin (GB12200) at dilution of 1: 4000

Lane 1: HeLa cell lysate

Lane 2: Jurkat cell lysate

Lane 3: 293T cell lysate

Lane 4: NIH3T3 cell lysate

Lane 5: C6 cell lysate

Lane 6: Mouse lung tissue lysate

Lane 7: Mouse brain tissue lysate

Lane 8: Rat lung tissue lysate

Lane 9: Rat brain tissue lysate

Immunofluorescent analysis of paraformaldehyde-fixed human kidney using alpha Tubulin (GB12200) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed human fibrogenic tumor using alpha Tubulin (GB12200) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using 

alpha Tubulin (GB12200) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using 

alpha Tubulin (GB12200) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed rat lung using 

alpha Tubulin (GB12200) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using 

alpha Tubulin (GB12200) at dilution of 1: 2000

Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using 

alpha Tubulin (GB12200) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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