Anti -RB-1 Rabbit pAb

價格：在線咨詢

貨號：

DRF101235

產品規(guī)格：

100 μL

Applications：

IHC/IF

Species：

M/R,M/R

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產品詳情操作說明注意事項

Product Information

Description	RB-1 rabbit polyclonal
Protein full name	Retinoblastoma-associated protein
Synonyms	pRb, Rb, pp105, Rb1
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse RB-1
Isotype	IgG
Purity	Affinity purification
Subcellular location	Nucleus
Uniprot ID	P13405, P33568

Applications

Applications	Species	Dilution	Positive Tissue
IHC	Mouse, Rat	1: 700-1: 1400	brain, kidney, lung, skeletal muscle
IF	Mouse, Rat	1: 500-1: 2500	brain, kidney, lung, skeletal muscle

Background

RB1 belongs to the retinoblastoma protein family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1/H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by Smarxa4/Brg1 by recruiting a histone deacetylase complex to the c-FOS promoter. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Images

	Immunohistochemistry analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1400
	Immunohistochemistry analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1400
	Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1000
	Immunofluorescent analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

凡合肥萬物生物科技有限公司銷售的產品均有嚴格的質量保證。如發(fā)現(xiàn)產品存在包裝規(guī)格疑問，請于收貨之日起七日內向我公司總部或當?shù)剞k事處提出，并請保證該產品，以備本公司進行核對檢驗。如果發(fā)現(xiàn)有質量問題，請于收貨后一月內保留產品50%以上的量，以便本公司回收產品進行質檢，確認產品質量。如逾期，則視為已經對本公司產品進行驗收。聲明：如發(fā)生產品索賠事宜，本公司僅在產品價格范圍內酌情賠償，恕不接受超出產品價格范圍之賠償。客戶在使用過程中有任何技術問題可以撥打技術售后服務電話我們隨時給予解答。

合肥萬物生物科技有限公司

客服熱線：400-1016-218

QQ：355185756

地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室