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Anti -Ki67 Rabbit pAb

價(jià)格: 在線咨詢(xún)
貨號(hào):
DRF10167
產(chǎn)品規(guī)格:
100 μL
Applications:
WB
Species:
H/M
購(gòu)買(mǎi)數(shù)量:
產(chǎn)品詳情 操作說(shuō)明 注意事項(xiàng)

Product Information



Description

Ki67 rabbit polyclonal

Protein full name

Proliferation marker protein Ki-67

Synonyms

MKI67, marker of proliferation Ki-67, KIA, MIB-, MIB-1, PPP1R105

Immunogen Recombinant protein corresponding to Mouse Ki67
Isotype IgG
Purity Affinity purification
Predicted MW. 359KDa
Observed MW.

359KDa

Subcellular location

Nucleus,nucleolus

Uniprot ID E9PVX6




Applications



Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse

1:300-1:600

Hela,HepG2,A431,liver,stomach,colon





Background



Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity).




Images



GB11030.png

Western blot analysis of Ki67 (GB11030) at dilution of 1:600.

Lane 1:Hela cell lysate

Lane 2:HepG2 cell lysate

Lane 3:A431 cell lysate

Lane 4:Human liver cancer tissue lysate

Lane 5:Human stomach cancer tissue lysate

Lane 6:Mouse colon tissue lysate





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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