Anti -PCNA Rabbit pAb

價格：在線咨詢

貨號：

DRF10151

產(chǎn)品規(guī)格：

100 μL

Applications：

WB/IHC/IF

Species：

H,H/M,H/M

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	PCNA rabbit polyclonal
Protein full name	Proliferating cell nuclear antigen
Synonyms	PCNA, ATLD2, proliferating cell nuclear antige
Immunogen	Recombinant protein corresponding to Mouse PCNA
Isotype	IgG
Purity	Affinity purification
Predicted MW.	29 kDa
Observed MW.	36 kDa
Subcellular location	Nucleus
Uniprot ID	Q12004, P17918, P04961

Applications

applications	species	dilution	Positive tissue
WB	Human	1: 500-1: 2000	HeLa, HepG2, MCF7, liver cancer
IHC	Human, Mouse	1: 1000-1: 2000	lung cancer, heart
IF	Human, Mouse	1: 400-1: 2000	lung cancer, testis, ovary cancer

Background

Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.

Images

	Western blot analysis of PCNA (GB11010-1) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: MCF-7 cell lysate Lane 3: 293T cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Mouse colon tissue lysate Lane 8: Rat testis tissue lysate Lane 9: Rat colon tissue lysate
	Immunohistochemistry analysis of paraffin-embedded human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000
	Immunohistochemistry analysis of paraffin-embedded mouse heart using PCNA (GB11010-1) at dilution of 1: 2000
	Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using PCNA (GB11010-1) at dilution of 1: 2000
	Immunofluorescent analysis of paraformaldehyde-fixed human ovary cancer using PCNA (GB11010-1) at dilution of 1: 2000
	Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using PCNA (GB11010-1) at dilution of 1: 2000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

凡合肥萬物生物科技有限公司銷售的產(chǎn)品均有嚴(yán)格的質(zhì)量保證。如發(fā)現(xiàn)產(chǎn)品存在包裝規(guī)格疑問，請于收貨之日起七日內(nèi)向我公司總部或當(dāng)?shù)剞k事處提出，并請保證該產(chǎn)品，以備本公司進(jìn)行核對檢驗。如果發(fā)現(xiàn)有質(zhì)量問題，請于收貨后一月內(nèi)保留產(chǎn)品50%以上的量，以便本公司回收產(chǎn)品進(jìn)行質(zhì)檢，確認(rèn)產(chǎn)品質(zhì)量。如逾期，則視為已經(jīng)對本公司產(chǎn)品進(jìn)行驗收。聲明：如發(fā)生產(chǎn)品索賠事宜，本公司僅在產(chǎn)品價格范圍內(nèi)酌情賠償，恕不接受超出產(chǎn)品價格范圍之賠償?？蛻粼谑褂眠^程中有任何技術(shù)問題可以撥打技術(shù)售后服務(wù)電話我們隨時給予解答。

合肥萬物生物科技有限公司

客服熱線：400-1016-218

QQ：355185756

地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學(xué)科技園A401室