Anti -PHD2 / prolyl hydroxylase Rabbit pAb

價格：在線咨詢

貨號：

KT012020

產(chǎn)品規(guī)格：

100 μL

Applications：

IHC/IF

Species：

MR/MR

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	PHD2 rabbit polyclonal
Protein full name	Egl nine homolog 1
Synonyms	Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20, Egln1
Immunogen	Recombinant protein corresponding to Mouse PHD2
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm. Nucleus
Uniprot ID	Q91YE3, P59722

Applications

Applications	Species	Dilution	Positive Tissue
IHC	Mouse, Rat	1: 850-1: 2000	brain, heart
IF	Mouse, Rat	1: 500-1: 1000	brain, heart

Background

Prolyl hydroxylase 2(PHD2) is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402), Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.

Images

	Immunohistochemistry analysis of paraffin-embedded mouse brain using PHD2 (GB111030) at dilution of 1: 1700
	Immunohistochemistry analysis of paraffin-embedded mouse heart using PHD2 (GB111030) at dilution of 1: 1700
	Immunohistochemistry analysis of paraffin-embedded rat brain using PHD2 (GB111030) at dilution of 1: 1700

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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