Anti -Lck Rabbit pAb

價格：在線咨詢

貨號：

DRF101770

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

品牌：

萬物

購買數(shù)量：

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產(chǎn)品詳情操作說明注意事項

Product Information

Description	Lck rabbit polyclonal
Protein full name	Proto-oncogene tyrosine-protein kinase LCK
Synonyms	Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, p56-LCK, Lck, Lsk-t
Immunogen	Recombinant protein corresponding to Mouse Lck
Isotype	IgG
Purity	Affinity purification
Predicted MW.	58 kDa
Observed MW.	58 kDa
Subcellular location	Cell membrane, Cytoplasm
Uniprot ID	P06239, P06240, Q01621

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	thymus, lymph nodes, spleen
IHC	Mouse, Rat	1: 1500-1: 4000	lung, lymph, spleen, thymus
IF	Mouse, Rat	1: 1500-1: 3000	lung, lymph, spleen

Background

LCK is a member of the Src family of protein tyrosine kinases (PTKs). This protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules.

Images

	Western blot analysis of Lck (GB111091) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: Mouse thymus tissue lysate Lane 3: Mouse lymph nodes tissue lysate Lane 4: Mouse spleen tissue lysate Lane 5: Rat thymus tissue lysate Lane 6: Rat lymph nodes tissue lysate Lane 7: Rat spleen tissue lysate
	Immunohistochemistry analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900
	Immunohistochemistry analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900
	Immunohistochemistry analysis of paraffin-embedded rat lymph using Lck (GB111091) at dilution of 1: 1900
	Immunohistochemistry analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 1900
	Immunohistochemistry analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 1900
	Immunohistochemistry analysis of paraffin-embedded mouse thymus using Lck (GB111091) at dilution of 1: 1900
	Immunohistochemistry analysis of paraffin-embedded rat thymus using Lck (GB111091) at dilution of 1: 1900
	Immunofluorescent analysis of paraffin-embedded mouse lung using Lck (GB111091) at dilution of 1: 1900
	Immunofluorescent analysis of paraffin-embedded rat lung using Lck (GB111091) at dilution of 1: 1900
	Immunofluorescent analysis of paraffin-embedded mouse lymph using Lck (GB111091) at dilution of 1: 1900
	Immunofluorescent analysis of paraffin-embedded mouse spleen using Lck (GB111091) at dilution of 1: 3000
	Immunofluorescent analysis of paraffin-embedded rat spleen using Lck (GB111091) at dilution of 1: 3000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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