Anti -6 X His Tag Rabbit pAb

價格：在線咨詢

貨號：

DRF10126

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

品牌：

萬物

購買數(shù)量：

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產(chǎn)品詳情操作說明注意事項(xiàng)

Product Information

Description	6 X His Tag rabbit polyclonal
Synonyms	6His, 6His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His probe, His Tag, His-probe, HISTIDINE TAGGED
Immunogen	KLH conjugated Synthetic peptide corresponding to HHHHHH (6* His)
Isotype	IgG
Purity	Affinity purification

Applications

Applications	Species	Dilution	Positive Tissue
WB	--	1: 500-1: 1000	His-Tag fusion protein

Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Images

Western blot analysis of 6*His Tag (GB111251) at dilution of 1: 1000
Lane 1, 2, 3, 6, 7: The different His-Tag fusion proteins
Lane 4, 5: The different GST-Tag fusion proteins

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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