Anti -Flag Tag ( DYKDDDDK Tag ) Mouse mAb

價(jià)格：在線咨詢

貨號(hào)：

DRF101431

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

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購(gòu)買(mǎi)數(shù)量：

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產(chǎn)品詳情操作說(shuō)明注意事項(xiàng)

Product Information

Description	Flag Tag ( DYKDDDDK Tag ) mouse monoclonal
Synonyms	DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG
Immunogen	KLH conjugated Synthetic peptide corresponding to DYKDDDDK
Isotype	IgG
Purity	Affinity purification

Applications

Applications	Species	Dilution	Positive Tissue
WB	--	1: 500-1: 1000	transfected 293T cells

Background

The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Images

Western blot analysis of FLAG Tag (GB12938)

at dilution of 1: 1000

Lane 1: 293T cells transfected with vector overexpressing Flag- tag fusion protein

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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