Anti -GAD65 Mouse mAb

價(jià)格：在線咨詢

貨號(hào)：

DRF101412

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

品牌：

萬(wàn)物

購(gòu)買數(shù)量：

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產(chǎn)品詳情操作說(shuō)明注意事項(xiàng)

Product Information

Description	GAD65 mouse monoclonal
Protein full name	Glutamate decarboxylase 2
Synonyms	GAD2, GAD65, glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, DCE 2, Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.
Immunogen	Recombinant protein corresponding to Mouse GAD65
Isotype	IgG
Purity	Affinity purification
Predicted MW.	65 kDa
Observed MW.	65 kDa
Subcellular location	Cell membrane, Cytoplasm
Uniprot ID	Q05683, P48320

Applications

Applications	Species	Dilution	Positive Tissue
WB	Mouse, Rat	1: 500-1: 1000	brain, cerebellum, hippocampus
IHC	Mouse, Rat	1: 1000-1: 2000	brain
IF	Mouse, Rat	1: 500-1: 2000	brain

Background

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Images

Western blot analysis of GAD65 (GB12562) at dilution of 1: 2000.

Lane 1: Human brain tissue lysate

Lane 2: Mouse brain tissue lysate

Lane 3: Mouse cerebral cortex tissue lysate

Lane 4: Mouse hippocampus tissue lysate

Lane 5: Mouse cerebellum tissue lysate

Lane 6: Rat brain tissue lysate

Lane 7: Rat cerebral cortex tissue lysate

Lane 8: Rat hippocampus tissue lysate

Lane 9: Rat cerebellum tissue lysate

Immunohistochemistry analysis of paraffin-embedded mouse brain using GAD65 (GB12562) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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