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Anti -Vimentin Mouse mAb
價(jià)格：
在線咨詢
貨號(hào)：
DRF101369
產(chǎn)品規(guī)格：
100 μL
Applications：
Species：
購(gòu)買數(shù)量：
產(chǎn)品詳情
操作說(shuō)明
注意事項(xiàng)
Product Information
|
Description |
Vimentin mouse monoclonal |
|
Protein full name |
Vimentin |
|
Synonyms |
VIM, CTRCT30, HEL113, vimentin |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse vimentin |
|
Isotype |
IgG |
|
Predicted MW. |
57 kDa |
|
Observed MW. |
57 kDa |
|
Purity |
Affinity purification |
|
Subcellular location |
|
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
Human, Mouse, Rat |
1:1000-1: 2000 |
lung |
|
IHC |
Mouse, Rat |
1: 500-1: 1000 |
lung cancer, colon, stomach, stomach cancer |
|
IF |
Mouse, Rat |
1: 500-1: 1000 |
NIH/ 3T3 |
Background
Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
Images
|
Western blot analysis of Vimentin (GB12192) at dilution of 1: 1000. |
|
Immunofluorescence analysis of mouse NIH/ 3T3 cells using Vimentin (GB12139) at dilution of 1: 1000 A: DAPI nuclear stain ( blue ) B: Anti- Vimentin ( red ) C: Merge |
|
|
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Vimentin (GB12192) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human colon using Vimentin (GB12192) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human stomach using Vimentin (GB12192) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human stomach cancer using Vimentin (GB12192) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬(wàn)物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室